By Carol Pugliano-Martin, Carol Pugliano

ISBN-10: 059018945X

ISBN-13: 9780590189453

Flip your school room right into a readers' theater with this pleasant selection of brief, easy performs on subject matters young ones adore-pets, dinosaurs, area, wasting a enamel, birthday events, making new neighbors, going to college, and lots of extra. those full of life performs contain lovely illustrations that aid the textual content in addition to rhymes, repition, and predictable language to aid bolster younger kid's interpreting and oral language talents. Comes whole with instructing suggestions and cross-curricular extensions. to be used with Grades K-1.

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Van Putten, J. P. , Wetzler, L. , Parsons, N. , Cole, J. A. & Smith, H. 1996 Functional characterization of a sialyltransferase deficient mutant of Neisseria gonorrhoeae. Infect. Immun. 64, 3374-3378. Gillaspy, A. , Hickmon, S. , Skinner, R. , Thomas, T. , Nelson, C. L. & Smeltzer, M. S. 1995 Role of the accessory gene regulator (agr) in pathogenesis of staphylococcal osteomyelitis. Infect. Immun. 63, 3373-3380. Gotschlich, E. , Seiff, M. , Blake, M. S. & Koomey, M. 1987 Porin protein of Neisseria gonorrhoeae: cloning and gene structure.

To confirm that the regulator is involved, expression of its genes should be detected in vivo; deletion of the genes should reduce virulence; and relevant environmental parameters should be present at the level at which they are effective in vivo. The IVET method did not detect expression of the cholera toxin gene nor other ToxR/ToxS regulated genes in the intestines of infected mice (Camilli & Mekalanos 1995); but STM in the same model detected mutants with insertions in tcp and toxT (Chiang &: Mekalanos 1995).

The recent development of techniques that permit stress responses to be studied directly in the in vivo situation has accelerated the pace of this field. ) and others that play a more general or 'global' role. This report deals with regulatory mechanisms of the latter class. It is concerned with determinants of the topology of bacterial DNA, each of which has been shown in vitro to be capable of modulating the transcriptional profile of the cell. These are DNA gyrase, a type II topoisomerase that introduces negative supercoils into DNA, an activity that is unique to prokaryotes; DNA topoisomerase I, an enzyme that relaxes DNA and which acts antagonistically to gyrase; integration host factor (IHF), a sequence-specific DNA-binding protein that places 1808 bends into DNA and modulates the function of many bacterial promoters, as well as influencing transposition, site-specific recombination, DNA replication and other DNA transactions; H-NS, a nucleoid-associated protein that binds DNA in a sequence-independent manner (it is thought to recognize and bind at, or close to, regions of intrinsic curvature in DNA) and can regulate transcription from a large number of promoters (almost always negatively) as well as influencing other DNA reactions such as site-specific recombination.

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25 Just-Right Plays for Emergent Readers: Reproducible Thematic With Cross-Curricular Extension Activities by Carol Pugliano-Martin, Carol Pugliano

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