By J. P. F. D'Mello

ISBN-10: 085199654X

ISBN-13: 9780851996547

ISBN-10: 0851997953

ISBN-13: 9780851997957

*Fully up to date from first version
*Includes a much broader diversity of animals
*Covers either farm and significant other animals

Amino acid metabolism and meals of livestock is still an lively sector of study. besides the fact that, because the booklet of the 1st version, as Amino Acids in Farm Animal Nutrition (1994), there's now a necessity take into consideration advances within the amino acid nutrients of a much broader variety of animals, together with significant other animals. during this re-creation, the editor has retained chapters offering power to the 1st model, whereas introducing authors with new principles and imaginative and prescient, in addition to chapters on different animals, resembling cats and canine. The publication is thematically based and comprises chapters of an introductory and common nature with purposes to quite a lot of animal species, views, and incorporated species-related sections, together with pigs, fowl, ruminants and different animals

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Example text

Liu (1994) tested the recovery of 14 amino acid standards added to a feed hydrolysate and found between 92 and 112% with a mean recovery of 98%, compared with IEC the recovery was 93–110%, except for threonine (85%) and arginine (117%). Indeed, he and some other authors heated the derivatization mixtures for 10 min at 50–55°C in sealed autosampler vials in a reaction block in order to finish the derivatization procedure. Liu et al. (1995) tested the methodology thoroughly in food and feed samples, ruling out interference from salt in neutralized hydrolysates.

As the work is done in different matrices, analysis by IEC and postcolumn ninhydrin derivatization is preferable to HPLC after precolumn derivatization. Check for compound feed quality Declarations of amino acid concentrations in commercial animal feedingstuffs are compulsory in many countries and are monitored by means of official amino acid analysis. e. to check ration formulation and production. Of particular concern is the accurate and homogeneous incorporation of the supplemented amino acids as they account for a considerable proportion of the total content and have therefore a major impact on animal performance.

1996). However, on comparing this procedure with HPLC analysis, the simple operation and greater precision 19 of the HPLC method is emphasized. The general acceptance of HPLC has therefore led to the virtual disappearance of the previously widespread spectrophotometric tryptophan methods. Molnár-Perl (1997) has compiled a review of methods of tryptophan analysis in peptides and proteins, including descriptions of techniques not normally used in feedingstuffs, such as acid or enzymatic hydrolysis, measurement by gas chromatography or derivative spectroscopy whithout chemical derivatization.

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Amino acids in animal nutrition by J. P. F. D'Mello


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